LOCUS pLenti CMV/TO GFP-Zeo DEST (719-1) 10150 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION 3rd gen lentiviral Gateway destination vector, expression, CMV/TO
promoter, GFP-Zeo.
ACCESSION .
VERSION .
KEYWORDS pLenti CMV/TO GFP-Zeo DEST (719-1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10150)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
REFERENCE 2 (bases 1 to 10150)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10150
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 26..130
/gene="bla"
/label=AmpR promoter
CDS 131..991
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(349..368)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1162..1750
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1651..1670
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1904..1921
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2038..2059
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2074..2104
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2112..2128
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2117..2139
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2136..2152
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2136..2152
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2171..2191
/label=T3
/note="T3 promoter, forward primer"
promoter 2173..2191
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2219..2445
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 2446..2626
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2673..2798
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3291..3524
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 3709..3753
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 4020..4136
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 4462..4665
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 4667..4685
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4688..4706
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4747..4871
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 4896..4926
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 4980..5639
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(5046..5065)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 5981..6286
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 6165..6184
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(6327..6451)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 6482..7070
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6535..6555)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6953..6964)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
enhancer 7145..7448
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 7449..7652
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 7691..7700
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 7697..8413
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQRDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(7742..7763)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(8003..8022)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 8350..8371
/label=EGFP-C
/note="EGFP, forward primer"
CDS 8426..8794
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
LTR 8887..9120
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 9192..9313
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9229..9248)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9283..9302
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 9353..9488
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9415..9434
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(9508..9527)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(9509..9527)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(9537..9554)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(9537..9553)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9546..9568)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 9695..10150
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9782..9801)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9992..10013
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
4141 ataagcttgg gagttccgct tacataactt acggtaaatg gcccgcctgg ctgaccgccc
4201 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
4261 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
4321 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
4381 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
4441 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
4501 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
4561 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
4621 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
4681 agagatctcc ctatcagtga tagagatcgt cgactagtcc agtgtggtgg aattctgcag
4741 atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
4801 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
4861 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
4921 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
4981 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
5041 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
5101 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
5161 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
5221 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
5281 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
5341 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
5401 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
5461 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
5521 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
5581 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
5641 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
5701 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
5761 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
5821 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
5881 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
5941 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
6001 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
6061 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
6121 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
6181 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
6241 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
6301 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
6361 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
6421 tttctcgttc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
6481 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
6541 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
6601 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
6661 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
6721 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
6781 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
6841 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
6901 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
6961 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
7021 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct
7081 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
7141 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
7201 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
7261 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
7321 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
7381 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
7441 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
7501 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
7561 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
7621 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
7681 gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
7741 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
7801 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
7861 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
7921 ccccgaccac atgaagcagc gcgacttctt caagtccgcc atgcccgaag gctacgtcca
7981 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
8041 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
8101 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
8161 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
8221 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
8281 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
8341 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
8401 cgagctgtac aagtccggac tcaccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
8461 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
8521 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
8581 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
8641 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
8701 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
8761 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt taaatggtac
8821 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
8881 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
8941 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
9001 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
9061 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
9121 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
9181 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
9241 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
9301 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
9361 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
9421 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
9481 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
9541 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
9601 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
9661 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
9721 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
9781 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
9841 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
9901 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
9961 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
10021 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
10081 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
10141 cttacaattt
//